We've preset your country and language selections based on your location. Select a different country or language below.
Messenger Wiwsi Handbags Lady Tote Casual Bags Party Women Gift Female Fashion ZSESHxPw Messenger Wiwsi Handbags Lady Tote Casual Bags Party Women Gift Female Fashion ZSESHxPw Messenger Wiwsi Handbags Lady Tote Casual Bags Party Women Gift Female Fashion ZSESHxPw Messenger Wiwsi Handbags Lady Tote Casual Bags Party Women Gift Female Fashion ZSESHxPw
Your Cart 0 items
     

Messenger Wiwsi Handbags Lady Tote Casual Bags Party Women Gift Female Fashion ZSESHxPw

high-quality blocking reagent eliminates cross-reactivity

MAXblock™ Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.

 
Name Format Cat No. Price  
MAXblock™ Blocking Medium 150 ml 15252 $135 Buy Now
MAXpack™ Immunostaining Media Kit 1 kit 15251 $350 Buy Now
 
 
Figure 1: MAXblock blocking efficacy.

Methanol-fixed HeLa cells grown on a coverslip were blocked overnight with MAXblock (A), 5% non-fat dry milk (C), 5% BSA (D) or Thermo-Fisher Pierce Sea Block (E). Cells were then incubated with a fluorescent anti-rabbit secondary antibody at a dilution of 1:250 (far above the recommended dilution to demonstrate the effectiveness of MAXblock), washed with MAXwash and mounted with DAPI. Any observed signal (pseudo-colored red) is caused by non-specific binding of the secondary due to incomplete blocking. Note that no staining is observed in panel A, demonstrating the effectiveness of MAXblock. (Panel B is a 150% overexposure of the fluorescent channel of the MAXblock slide, which shows cell position and demonstrates that antibody binding is barely detectable above background.) Experiments with an anti-mouse secondary gave nearly identical results.

Get the exact immunofluorescence reagents you need

For your convenience, MAXblock is also offered as a component in the MAXpack™ Immunostaining Media Kit, which also contains MAXbind™ Staining Medium and MAXwash™ Washing Medium.

Contents & Storage

150 ml of MAXblock Blocking Medium. The MAXpack™ Immunostaining Media Kit contains 150 ml MAXblock, 250 ml of MAXbind™ and 1000 ml of MAXwash™. Store all media at 4°C; all reagents are guaranteed stable for 6 months when stored properly.